Date: May 30, 2009
Circuit: Autodromo del Mugello
Location: Italy
Circuit Length: 5.245 km
Width: 14 m
Pole Position: Right
Longest Straight: 1.141 m
Left Corners: Six
Right Corners: Nine
| Pos. | Rider Name | Team | Time |
|---|---|---|---|
| 1 | Bradley SMITH | Bancaja Aspar Team 125cc | 40'09.523 |
| 2 | Nicolas TEROL | Jack & Jones Team | +0.216 |
| 3 | Julian SIMON | Bancaja Aspar Team 125cc | +7.114 |
| 4 | Pol ESPARGARO | Derbi Racing Team | +11.829 |
| 5 | Marc MARQUEZ | Red Bull KTM Motosport | +12.315 |
| 6 | Johann ZARCO | WTR San Marino Team | +14.605 |
| 7 | Scott REDDING | Blusens Aprilia | +15.305 |
| 8 | Stefan BRADL | Viessmann Kiefer Racing | +22.255 |
| 9 | Lorenzo SAVADORI | Fontana Racing | +22.392 |
| 10 | Sandro CORTESE | Ajo Interwetten | +29.239 |
| 11 | Sergio GADEA | Bancaja Aspar Team 125cc | +36.359 |
| 12 | Lorenzo ZANETTI | Ongetta Team I.S.P.A | +36.444 |
| 13 | Randy KRUMMENACHER | Degraaf Grand Prix | +36.507 |
| 14 | Jonas FOLGER | Ongetta Team I.S.P.A | +36.581 |
| 15 | Takaaki NAKAGAMI | Ongetta Team I.S.P.A | +36.636 |
| 16 | Esteve RABAT | Blusens Aprilia | +36.834 |
| 17 | Luigi MORCIANO | Junior GP Racing Dream | +49.870 |
| 18 | Simone CORSI | Jack & Jones Team | +50.419 |
| 19 | Dominique AEGERTER | Ajo Interwetten | +1'05.900 |
| 20 | Riccardo MORETTI | Elligi Racing | +1'06.019 |
| 21 | Davide STRIPE | CRP Racing | +1'20.225 |
| 22 | Lukas SEMBERA | Matteoni Racing | +1'21.946 |
| 23 | Jasper IWEMA | Racing Team Germany | +1'43.074 |
| 24 | Gennaro SABATINO | Junior GP Racing Dream | 1 Lap |
| 25 | Luca VITALI | CBC Corse | 1 Lap |
